This slot is intended for N-term tags, such as affinity tags, solubilization tags or localization tags. This slot is flanked by a NdeI restriction site (CATATG) at the 5' that encodes for the start Methionine so you don't need to place one. In addition, this slot is flanked by a Kpn2I at the 3' (TCCGGA) that encodes for Ser-Gly linker to allow in-frame fusion protein with the downstream slot (your custom gene).
mRFP1_PEO (MGEC8J2)
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Current choice for
“N-terminus domain”
CDS of a monomeric red fluorescent protein optimized for the expression in E. coli through Doulix's protein expression optimization algorithm. The sequence contains no start nor stop codon. The original non-optimized sequence is available in biomodule M1LCLRL.
The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.