The biomodule carries all the elements to drive RNA transcription under the control of arabinose in E. coli. The coding sequence of the AraC repressor is driven by the pC promoter directed in the opposite direction of the arabinose-inducible promoter pBAD. AraC gene transcription is interrupted by a transcription terminator as in plasmid series pGR (doi.org/10.1038/nmeth.2515) and AraC coding sequence was optimized to remove all the restriction sites incompatible with DNAmate assembly. The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by arabinose.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.