The biomodule carries all the elements to drive RNA transcription under the control of phloretin in E. coli. An operator for the TetR-family factor PmeR was evolved to allow the induction of gene expression by phloretin (doi.org/10.1093/nar/gkz772). The coding sequence of the PmeR repressor is driven by a constitutive promoter directed in the opposite direction of the phloretin-inducible promoter Ppme. PmeR gene transcription is interrupted by a transcription terminator and PmeR coding sequence was optimized to remove all the restriction sites incompatible with DNAmate assembly. The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by phloretin.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.