T9
This Biomodule encodes for a yeast terminator of the MIAMI Project collection. This terminator was characterized in vivo in S.cerevisiae [1] using the protocol described in [2]. Briefly, the terminator was cloned downstream the MIAMI Standard reporter gene cassette [3] and integrated into S.cerevisiae genome by EasyClone marker-free integration as described in [4]. Terminator’s strength was assessed in vivo over 24 hours and results re-scaled using the standard operating procedure described in [5].
MIAMI collection was designed by members of the MIAMI consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 814645.
Reference: [1] S.cerevisiae CEN.PK2-1C [2] MIAMI's Standard Operating Procedures [3] MIAMI - Standard reporter gene cassette [4] Mathew M Jessop‐Fabre et al. EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9. Biotechnol J. 2016 Aug; 11(8): 1110–1117.Link [5] Whole cell fluorescence reading Ex485nm/Em530nm with plate reader
- Part ID 6YKC5YP
- Name T9
- Alternative Name -
- Version T9
- Division UNA
- Parent Sequence -
- Collection MIAMI project
- Part Status Valid
- Public check_circle
- Certified highlight_off
- Length 39
- Source Strain -
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Suitable for
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- Registry Code -
- Contains
- Created by Jensen Lab – DTU
- Created at Oct. 13, 2020, 4:12 p.m.
- Last Edit Oct. 19, 2020, 10:26 a.m.