This slot is intended for coding sequences such enzymes or custom proteins. This slot is flanked by a Kpn2I restriction site (TCCGGA) at 5' that codifies for an upstream Ser-Gly linker and by a BamHI restriction site (GGATCC) at 3' that codifies for a downstream Gly-Ser linker. These linkers allow for the creation of an in-frame fusion protein with the coding sequences upstream (N-term) and downstream (C-term).
Double STOP codons (MJDCY28)
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“Your Custom gene”