This slot is intended for the 5' untranslated region (5'-UTR) such as regulatory elements or the Shine-Dalgarno sequence. This slot carries a START codon (ATG) at the 3' terminus so that you can place your coding sequence downstream in Slot C. Please choose among our broad choice of 5'-UTR or design your custom sequence from scratch by clicking the 'Create New' tile.
RBS BBa_B0032 + TAC (699CGKV)
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“5' untranslated region”
The biomodule carries the RBS sequence of registry part BBa_B0032 and a 3-nucleotide spacer to provide the correct distance between RBS and start codon in DNAmate assemblies. For this purpose, the biomodule should be flanked by DNAmate's hinges B and C corresponding to AflII and NdeI restriction cut sites respectively.
The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.