The biomodule carries all the elements to drive RNA transcription under the control of anhydrotetracycline (aTc) in E. coli. The coding sequence of the TetR repressor is driven by a constitutive promoter directed in the opposite direction of the aTc-inducible promoter PTet. TetR gene transcription is interrupted by a transcription terminator and TetR coding sequence was optimized to remove all the restriction sites incompatible with DNAmate assembly. The biomodule shall be placed right upstream of a 5' UTR sequence of a gene of interest to have the expression controlled by aTc.

The ACDC collection was designed by members of the ACDC consortium supported by the EU Horizon 2020 research and innovation programme. Grant agreement No 824060. https://cordis.europa.eu/project/id/824060.